Nicotine and its metabolites: radioimmunoassay for. gamma. -(3-pyridyl)-. gamma. -oxo-N-methylbutyramide. [/sup 125/I]
Journal Article
·
· Arch. Biochem. Biophys.; (United States)
A radioimmunoassay has been developed for the nicotine metabolite ..gamma..-(3-pyridyl)-..gamma..-oxo-N-methylbutyramide. The sensitivity and specificity of the assay allow the determination of this compound at the picomole level in the presence of several structurally related molecules including nicotine and seven other nicotine metabolites. The assay has been used to characterize the enzyme system present in rabbit liver extract responsible for the conversion of cotinine to the oxoamide, and to measure oxoamide levels in urine, sera, and amniotic fluid of smokers. High-pressure liquid chromatography was used as an independent method to follow the enzymatic oxidation and in conjunction with the radioimmunoassay to analyze the urine samples. (auth)
- Research Organization:
- Brandeis Univ., Waltham, MA
- OSTI ID:
- 7364239
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 164:2; ISSN ABBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALOIDS
AMIDES
AMINES
ANIMALS
ANTIGENS
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
NICOTINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIDINES
PYRROLES
PYRROLIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALOIDS
AMIDES
AMINES
ANIMALS
ANTIGENS
AZINES
AZOLES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
NICOTINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIDINES
PYRROLES
PYRROLIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES