Ca/sup 2 +/-mobilizing actions of platelet-derived growth factor differ from those of bombesin and vasopressin in Swiss 3T3 mouse cells

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.16.5768· OSTI ID:5172501

Lopez-Rivas, A; Mendoza, S A; Nanberg, E; Sinnett-Smith, J; Rozengurt, E

Addition of the mitogenic peptides bombesin and vasopressin to quiescent Swiss 3T3 mouse cells increased the cytosolic Ca/sup 2 +/ concentration without any measurable delay. In contrast, there was a significant lag period (16 +/- 1.2 s) before platelet-derived growth factor (PDGF) increased cytosolic Ca/sup 2 +/ concentration. This lag was not diminished at high concentrations of either porcine or human PDGF. Similar results were obtained in 3T3 cells loaded with quin-2 or fura-2. The differences in the effects of bombesin, vasopressin, and PDGF on Ca/sup 2 +/ movements were also substantiated by measurements of /sup 45/Ca/sup 2 +/ efflux and of cellular /sup 45/Ca/sup 2 +/ content. Activation of protein kinase C by phorbol esters inhibited Ca/sup 2 +/ mobilization induced by either bombesin or vasopressin. In contrast, phorbol esters had no effect on PDGF-induced cytosolic Ca/sup 2 +/ concentration increase or acceleration of /sup 45/Ca/sup 2 +/ efflux. Finally, bombesin and vasopressin caused a rapid increase in the production of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate, whereas PDGF, even at a saturating concentration, exerted only a small effect. These results indicate that the signal transduction pathway activated by PDGF that lead to Ca/sup 2 +/ mobilization can be distinguished form those utilized by bombesin and vasopressin.

• Research Organization: Imperial Cancer Research Fund, London (England)
• OSTI ID: 5172501
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:16; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CALCIUM 45
CALCIUM ISOTOPES
CARCINOGENS
CATIONS
CHARGED PARTICLES
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERS
EVEN-ODD NUCLEI
FIBROBLASTS
HORMONES
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRANSFERASES
VASOPRESSIN
VERTEBRATES