EGF raises cytosolic Ca sup 2+ in A431 and Swiss 3T3 cells by a dual mechanism

Journal Article · Fri May 01 04:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90127-3· OSTI ID:6531079

Pandiella, A; Malgaroli, A; Meldolesi, J; Vicentini, L M ^[1]

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1. Univ. of Milano (Italy)

The changes in Ca{sup 2+} homeostasis and phosphoinositide hydrolysis induced by EGF were studied in human epidermoid carcinoma A431 cells both when attached to a substratum and after detachment and suspension. The cytosolic Ca{sup 2+} concentration was measured by the conventional fluorimetric technique, using the specific probe, quin2, as well as by a new microscopic technique in which single cells are investigated after loading with another probe, fura-2. EGF applied in the complete, Ca{sup 2+}-containing medium caused a rapid rise in the cytosolic {sup 45}Ca{sup 2+} concentration, that remained elevated for several minutes. In Ca{sup 2+}-free, EGTA-containing medium, part of this response persisted, as revealed by quin2 results in suspended cells and microscopic results with fura-2. These results, as well as additional microscopic fura-2 results in Swiss 3T3 fibroblasts, demonstrate that the Ca{sup 2+} signal elicited by EGF is due to two components: redistribution from an intracellular store and stimulated influx across the plasmalemma. This latter process was not detected in 3T3 cells treated with either PDGF or bombesin. It is therefore suggested that the {sup 45}Ca{sup 2+} influx effect of EGF is under the control of a separate, as yet unidentified mechanism.

• OSTI ID: 6531079
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 170:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
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ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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CALCIUM 45
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CALCIUM ISOTOPES
CARBOHYDRATES
CARCINOMAS
CELL CONSTITUENTS
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CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
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EVEN-ODD NUCLEI
FIBROBLASTS
FLUORESCENCE SPECTROSCOPY
GROWTH FACTORS
HOMEOSTASIS
INOSITOLS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MAN
MEMBRANE TRANSPORT
MEMBRANES
MITOGENS
MONOSACCHARIDES
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SPECTROSCOPY
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VERTEBRATES