Formation and metabolism of inositol 1,4,5 trisphosphate in human platelets
(/sup 3/H)Myo-inositol (1,4,5)trisphosphate ((1,4,5)IP/sub 3/), when added to lysed platelets, was rapidly converted to (/sup 3/H)inositol (1,3,4,5)tetrakisphosphate which was in turn converted to (/sup 3/H)inositol (1,3,4)trisphosphate. This result demonstrates that platelets have the same metabolic pathways for interconversion of inositol polyphosphates that are found in other cells. Labelling of platelets with (/sup 32/P)orthophosphate, followed by h.1.p.c. was used to measure thrombin-induced changes in the three inositol polyphosphates. Interfering compounds were removed by a combination of enzymatic and nonenzymatic techniques. (/sup 32/P)-(1,4,5)IP/sub 3/ was formed rapidly and reached its maximal level at about 4 sec. It was also rapidly degraded and was no longer detectable after 30-60 sec. Formation of (1,3,4,5)IP/sub 4/ was almost as rapid as that of (1,4,5)IP/sub 3/ and remained at detectable levels for a longer time. (1,3,4)IP/sub 3/ was formed after an initial lag and this isomer reached its maximal level that was ten-fold higher than that of (1,4,5)IP/sub 3/ at 30 sec. Comparison of the intracellular Ca/sup 2 +/ concentration as measured with fura-2 indicates that agents other than (1,4,5)IP/sub 3/ are responsible for the sustained maintenance of a high level of intracellular Ca/sup 2 +/. It is proposed that either (1,3,4)IP/sub 3/ or (1,3,4,5)IP/sub 4/ may also be Ca/sup 2 +/-mobilizing agents.
- Research Organization:
- Temple Univ., Philadelphia, PA
- OSTI ID:
- 6125160
- Report Number(s):
- CONF-870644-; TRN: 87-034063
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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INOSITOL
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DAYS LIVING RADIOISOTOPES
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550501* - Metabolism- Tracer Techniques