Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Activity of DNA-dependent DNA polymerase and aspartate--carbamoyl transferase after separation of rat marrow and spleen cells in an albumin density gradient under normal conditions, after irradiation and administration of DNA. [Gamma radiation]

Journal Article · · Radiobiology (USSR) (Engl. Transl.); (United States)
OSTI ID:5166723
;
Normal rat bone marrow and spleen cells, and cells taken at different times following irradiation (600 R) and administration of DNA were separated into nine fractions in an albumin density gradient. Radiation lowered DNA-dependent DNA polymerase and aspartate-carbamoyl transferase activity in cells of the fractions in which it was normally the highest. There was restoration of distribution of cells according to levels of enzymatic activity on the 15th to 20th post-radiation day. Administration of DNA did not affect the nature of postradiation changes in distribution of enzyme activity, but normalized DNA content in some fractions of bone marrow cells on the 5th, rather than 10th to 20th postradiation day.
OSTI ID:
5166723
Journal Information:
Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 17:5; ISSN RADBA
Country of Publication:
United States
Language:
English

Similar Records

BONE MARROW REGENERATION IN IRRADIATED-BLED MICE STUDIED BY THYMIDINE-H$sup 3$
Journal Article · Sat Nov 30 23:00:00 EST 1963 · Nuclear Hematology (England) Published in Nucl. Energy (Inst. Nucl. Eng.) · OSTI ID:4144548
Specialized DNA polymerases in lymphoid cells
Conference · Fri Dec 31 23:00:00 EST 1976 · Cold Spring Harbor Symp. Quant. Biol.; (United States) · OSTI ID:5146266
DYNAMICS OF HEMOPOIETIC PROLIFERATION IN MAN AND MICE STUDIED BY $sup 3$H- THYMIDINE INCORPORATION INTO DNA
Technical Report · Thu Oct 30 23:00:00 EST 1958 · OSTI ID:4308743

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
GAMMA RADIATION
IONIZING RADIATIONS
MAMMALS
METABOLISM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN CELLS
TRANSFERASES
VERTEBRATES