DYNAMICS OF HEMOPOIETIC PROLIFERATION IN MAN AND MICE STUDIED BY $sup 3$H- THYMIDINE INCORPORATION INTO DNA
human being with normal and abnormal hematopoiesis to study the kinetics of bone marrow cell proliferation. Thymidine is taken up by the nucleus only during DNA synthesis preparatory to cell division. Its intracellular location was determined and quantified by means of stripping film autoradiography and classical cytology. In the bone marrow of human beings with normal hemlabeling of primitive proliferating cells was seen within minutes. Maximal labeling of erythorocyte precursors the 3rd and 4td day. Maximal labeling of megakaryocytes was observed on the 5th day. Early labeling in the peripheral blood of large mononuclear cells occured early; the peak labeling of granulocytes occured on the 5th to 6th day. H/sup 3/ thymidine incubated with normal series; normal venous blood showed labeling only of a tern of H/sup 3/ thymidine uptake with in vitro incubation of bone marrow and venous blood from patients varied with different hematopoietic disorders. A preliminary analysis of the quantitative results in terms of the kinetics of hematopoietic cell proliferation in health and disease is made, and the requirements for resolution of the complex probelms involved are pointed out. (auth)
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.; National Institutes of Health, Bethesda, Md.
- NSA Number:
- NSA-12-014565
- OSTI ID:
- 4308743
- Report Number(s):
- A/CONF.15/P/840
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGY AND MEDICINE
BLOOD CIRCULATION
BLOOD FORMATION
BONE MARROW
CONFERENCE
CYTOLOGY
DISEASES
DNA
ERYTHROCYTES
FILMS
GRANULOCYTES
LEUCOCYTES
MAN
MEGAKARYOCYTES
METABOLISM
MICE
MITOSIS
MONOCYTES
NUCLEIC ACIDS
QUANTITY RATIO
RADIOAUTOGRAPHY
THYMIDINE
TRACER TECHNIQUES
TRITIUM
VARIATIONS