Increased granulocytic, erythrocytic, and megakaryocytic progenitors in myelofibrosis with myeloid metaplasia

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Am. J. Hematol.; (United States)

DOI:https://doi.org/10.1002/ajh.2830040204· OSTI ID:5827534

Chikkappa, G; Carsten, A L; Chanana, A D; Chandra, P; Cronkite, E P

Nucleated cells obtained from blood and/or bone marrow of patients with myelofibrosis with myeloid metaplasia (MMM) were cultured in diffusion chambers (DC) implanted into the peritoneal cavities of irradiated mice. A total of five blood studies and two bone marrow studies were performed using cells obtained from five patients. The DC were harvested at intervals from the host mice and the total and differential cellularity of DC contents were evaluated. The results obtained from MMM cultures were compared with those from similar cultures of blood cells and marrow cells of four and six normal individuals respectively. The proliferation and maturation of the granulocytic, erythrocytic, and megakaryocytic lines in MMM cultures occurred in an orderly fashion as they occur in vivo. The patterns of proliferation and maturation of the three cell lines in cultures after day 7 suggest that they primarily originate from progenitor cells. The numbers of granulocytes in the multiplicative pool, recognizable red cell precursors, and megakaryocytes recovered were significantly greater from the MMM cultures than those from the normal blood or marrow cultures. These results suggest that the blood and marrow cells of MMM patients have increased numbers of progenitors for granulocytes, erythrocytes and megakaryocytes.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5827534
• Journal Information: Am. J. Hematol.; (United States), Journal Name: Am. J. Hematol.; (United States) Vol. 4; ISSN AJHED
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
ERYTHROCYTES
HEMIC DISEASES
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
LEUKOCYTES
MAMMALS
MAN
MICE
PRECURSOR
PRIMATES
RODENTS
SOMATIC CELLS
STEM CELLS
VERTEBRATES