Macrocytic megakaryocytes in cultures of S1/S1/sup d/ bone marrow

Journal Article · Sat Dec 31 23:00:00 EST 1983 · Exp. Hematol.; (United States)

OSTI ID:5351985

Adrados, C; Ebbe, S; Phalen, E; Garbutt, P; Allan, C

The thrombocytopoietic system of S1/S1/sup d/ mice is characterized by macromegakaryocytosis and megakaryocytopenia, but the mechanisms responsible for reciprocal abnormalities of megakaryocyte number and size are unknown. These mice have a genetically determined abnormality of their hemopoietic microenvironment that can, in part, be reproduced as abnormal adherent stromal cells in bone marrow cultures. Cultures of bone marrow were therefore done to determine if the megakaryocytic abnormalities of S1/S1/sup d/ marrow would also be reproduced in them. Cultures composed entirely of S1/S1/sup d/ cells showed persistent macrocytosis of megakaryocytes when compared with cultures of normal +/+ marrow. At various times of culture, there were also reduced numbers of megakaryocytes, total cells, and granulocytes in the supernatants of S1/S1/sup d/ cultures. Mixed cultures of S1/S1/sup d/ and +/+ cells yielded inconclusive findings. The fact that macromegakaryocytosis occurred in cultures of S1/S1/sup d/ marrow strongly suggests that its in vivo determinants were, in part, reproduced in culture. 27 references, 5 figures, 2 tables.

• Research Organization: Lawrence Berkeley Lab, CA
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5351985
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 12; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DATA
ERYTHROCYTES
EXPERIMENTAL DATA
HEMATOPOIETIC SYSTEM
INFORMATION
LEUKOCYTES
MALFORMATIONS
MAMMALS
MATERIALS
MICE
NUMERICAL DATA
PATHOLOGICAL CHANGES
RODENTS
SIZE
SOMATIC CELLS
VERTEBRATES