Identification of an essential site for transcriptional activation within the human T-cell receptor. delta. enhancer

Journal Article · Fri Nov 01 04:00:00 EST 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.11.5671· OSTI ID:5116419

Redondo, J M; Pfohl, J L; Krangel, M S ^[1]

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1. Duke Univ., Durham, NC (United States)

A T-cell-specific transcriptional enhancer was previously identified within the J{sub {delta}3}-C{sub {delta}} intron of the human T-cell receptor (TCR) {delta} gene, and seven distinct binding sites for nuclear factors ({delta}E1 to {delta}E7) were defined by DNase I footprinting. In this study, conducted a detailed functional analysis of the various cis-acting DNA sequence elements of the enhancer and show that a 60-bp fragment encompassing {delta}E3 and {delta}E4 displays potent enhancer activity, as judged by its ability to activate transcription from the V{sub {delta}}1 promoter. They show that the interaction of nuclear factors with the {delta}E3 site is essential for enhancer activity. This element displays significant activity in the absence of additional segments of the enhancer. Further, methylation interference and in vitro mutagenesis identify a site within {delta}E3 that mediates the binding of two nuclear factors (NF-{delta}E3A and NF-{delta}E3C) and that is required for significant transcriptional activation by the enhancer. NF-{delta}E3C is ubiquitous and may be identical to a previously characterized {mu}E3-binding factor, NF-{delta}E3A is preferentially expressed in T lymphocytes, and the authors suggest that this factor may play the dominant role in transcriptional activation through the {delta}E3 site. One of three specific complexes formed with a {delta}E4 probe appears to be T-cell specific.

• OSTI ID: 5116419
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:11; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

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550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
DNA-ASE
ENZYMES
ESTERASES
GENE REGULATION
GENES
HYDROLASES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
METHYLATION
MOLECULAR BIOLOGY
MUTAGENESIS
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHODIESTERASES
PLASMIDS
POLYPEPTIDES
PRIMATES
PROTEINS
RECEPTORS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES