The first intron of the 4F2 heavy-chain gene contains a transcriptional enhancer element that binds multiple nuclear proteins

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.9.6.2588· OSTI ID:5822258

Karpinski, B A; Yang, L H; Cacheris, P; Morle, G D; Leiden, J M

The authors utilized the human 4F2 heavy-chain (4F2HC) gene as a model system to study the regulation of inducible gene expression during normal human T-cell activation. Previous studies have demonstrated that 4F2HC gene expression is induced during normal T-cell activation and that the activity of the gene is regulated, at least in part, by the interaction of a constitutively active 5'-flanking housekeeping promoter and a phorbol ester-responsive transcriptional attenuator element located in the exon 1-intron 1 region of the gene. They now report that 4F2HC intron 1 contains a transcriptional enhancer element which is active on a number of heterologous promoters in a variety of murine and human cells. This enhancer element has been mapped to a 187-base-pair RsaI-AluI fragment from 4F2HC intron 1. DNase I footprinting and gel mobility shift analyses demonstrated that this fragment contains two nuclear protein-binding sites (NF-4FA and NF-4FB) which flank a consensus binding site for the inducible AP-1 transcription factor. Deletion analysis showed that the NF-4FA, NF-4FB, and AP-1 sequences are each necessary for full enhancer activity. Murine 4F2HC intron 1 displayed enhancer activity similar to that of its human counterpart. Comparison of the sequences of human and murine 4F2HC intron 1s demonstrated that the NF-4FA, NF-4FB, and AP-1 sequence motifs have been highly conserved during mammalian evolution.

• Research Organization: Michigan Univ., Ann Arbor, MI (USA). Medical Center
• OSTI ID: 5822258
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 9:6; ISSN MCEBD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL PROLIFERATION
CHEMICAL BONDS
CHROMOSOME LOSSES
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
ESTERS
GENE AMPLIFICATION
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC MAPPING
GLOBULINS
HUMAN POPULATIONS
IMMUNOGLOBULINS
LEUKOCYTES
LOSSES
LYMPHOCYTES
MAMMALS
MAPPING
MATERIALS
MICE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHORBOL ESTERS
POPULATIONS
PROTEINS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
VERTEBRATES