Systematic binding analysis of the insulin gene transcription control region: Insulin and immunoglobulin enhancers utilize similar transactivators

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.6.2620· OSTI ID:7002966

Moss, L G; Moss, J B; Rutter, W J

The 5' regulatory region (-345 to +1) of the rat insulin I gene (Ins-I) was examined for binding to cellular factors with short oligodeoxynucleotide probes. Over 40 binding species were detected. The binding profiles were specific for each cell type studied. The authors characterized the factors binding two elements crucial for enhancer activity (the Nir and Far boxes) which bear sequence similarity to the ..mu..E1, ..mu..E2, and ..mu..E3 elements of the immunoglobin heavy-chain enhancer. The Nir box binds three cellular factors that display preferential affinities for ..mu..E1, ..mu..E2, or ..mu..E3 and the Far box binds two factors related to ..mu..E2 or ..mu..E3. The insulin gene enhancer was mutated at the Nir box element to reflect the sequences of ..mu..E1, ..mu..E2, or ..mu..E3. Ins-..mu..E2 was fully active, Ins-..mu..E3 was partially active, and Ins-..mu..E1 was inactive. Thus, factors similar or identical to nuclear factor NF-..mu..E1, NF-..mu..E2, or NF-..mu..E3 may play a role in the activity of the insulin gene enhancer.

• Research Organization: Hormone Research Institute and Dept. of Biochemistry and Biophysics, Univ. of California, San Francisco, CA (US)
• OSTI ID: 7002966
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:6; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL PATHWAYS
GENE OPERONS
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
GLOBULINS
GROWTH FACTORS
HORMONES
IMMUNOGLOBULINS
INSULIN
MAPPING
MITOGENS
NUCLEIC ACIDS
NUCLEOPROTEINS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
SOMATIC CELLS
TRANSCRIPTION