Activation and transcription by binding of NF-E1 (YY1) to a newly identified element in the first exon of the human DR{alpha} gene

Journal Article · Mon May 15 04:00:00 EDT 1995 · Journal of Immunology

OSTI ID:83480

Hehlgans, T; Strominger, J L ^[1]

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1. Harvard Univ., Cambridge, MA (United States)

A previously unrecognized element, located downstream of the start site of transcription in the first exon of the DR{alpha} gene, has been defined that enhances promoter activity up to eightfold in a position-dependent manner. Mutations in this DNA-binding site abolished binding of a nuclear factor in human B cell nuclear extract and decreased the activity of the DR{alpha} promoter to a basal level. Significant sequence homology of this element was found in the DNA of the DR{beta}, DP{alpha} and -{beta}, and DQ{alpha} genes, always located downstream of the transcriptional start site. The nuclear factor binds to the DR{alpha} and DP{alpha} element but not to the element in the DQ{alpha} gene. It was identified as NF-E1 (YY1). This protein, previously identified by its binding to the Ig K 3{prime} enhancer and the Ig heavy chain {mu}E1 site, thus also appears to be quite important in the regulation of MHC class II gene expression. 39 refs., 6 figs., 1 tab.

• OSTI ID: 83480
• Journal Information: Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 10 Vol. 154; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA
GENE MUTATIONS
GENES
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 6
IMMUNOGLOBULINS
OLIGONUCLEOTIDES
PROTEINS
TRANSCRIPTION