Aerobic glycolysis in bone: lactic acid production by rat calvaria cells in culture

Journal Article · Sun Aug 01 04:00:00 EDT 1976 · Am. J. Physiol.; (United States)

OSTI ID:5115680

Felix, R; Neuman, W F; Fleisch, H

Considerable data have been accumulated on aerobic glycolysis of intact bone preparations. To test whether aerobic glycolysis is a feature of the bone cells themselves or of the localized conditions within an intact bone, cells isolated from rat calvaria were cultured and the effect of several factors on lactate production was determined. These cells drastically decreased lactate production when the pH in the culture medium was lowered, changing from 100 to 20% for a pH shift from 7.4 to 6.75. L-lactate inhibited its own formation by 40% at 20 mM. Parathyroid hormone (PTH) (820 U/mg) at a concentration ranging from 0.2 to 5.0 U/ml stimulated slightly the lactate production in a log-linear response, the ratio treated over control changing from 1.1 to 1.3. The maximal stimulation is observed at pH 7.0. Isolated cells respond qualitatively the same as did intact calvaria. Quantitatively, there were significant differences: notably, a smaller response to parathyroid hormone and a higher sensitivity to lowered pH.

• Research Organization: Univ., Bern
• OSTI ID: 5115680
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 234:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BONE CELLS
BONE TISSUES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DECOMPOSITION
GLYCOLYSIS
HORMONES
HYDROXY ACIDS
IN VITRO
LACTIC ACID
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PH VALUE
PRODUCTION
RATS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES