Parathyroid hormone blocks the stimulatory effect of insulin-like growth factor-I on collagen synthesis in cultured 21-day fetal rat calvariae

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Bone; (USA)

DOI:https://doi.org/10.1016/8756-3282(90)90136-M· OSTI ID:5803013

Kream, B E; Petersen, D N; Raisz, L G ^[1]

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1. Univ. of Connecticut Health Center, Farmington (USA)

We examined the interaction of parathyroid hormone (PTH) and recombinant human insulin-like growth factor I (IGF-I) on collagen synthesis in 21-day fetal rat calvariae as assessed by measuring the incorporation of ({sup 3}H)proline into collagenase-digestible protein. After 96 hours of culture, 10 nM PTH antagonized the stimulation of collagen synthesis and partially blocked the increase in dry weight produced by 10 nM IGF-I. The effect of PTH to block IGF-I stimulated collagen synthesis was observed in the central bone of calvariae and was mimicked by forskolin and phorbol 12-myristate 13-acetate, but not by 1,25-dihydroxyvitamin D3, transforming growth factor-alpha or dexamethasone. Our data are consistent with the concept that the direct effect of PTH is to inhibit basal CDP labeling and fully oppose IGF-I stimulated CDP labeling. The finding that this effect of PTH is mimicked by forskolin and PMA suggests that this block in IGF-I stimulation of CDP labeling involves both cAMP and protein kinase C mediated pathways.

• OSTI ID: 5803013
• Journal Information: Bone; (USA), Journal Name: Bone; (USA) Vol. 11:6; ISSN BONED; ISSN 8756-3282
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
AMINES
AMINO ACIDS
AMP
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CARCINOGENS
COLLAGEN
CORTICOSTEROIDS
DEXAMETHASONE
ENZYMES
ESTERS
FETUSES
GLUCOCORTICOIDS
GROWTH FACTORS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LIPIDS
MAMMALS
MITOGENS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PREGNANES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RATS
REACTION KINETICS
RODENTS
SCLEROPROTEINS
SKELETON
SKULL
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES