Parathyroid hormone stimulation of collagenase secretion by isolated bone cells. [Rats]

Journal Article · Thu Feb 01 04:00:00 EST 1979 · Endocrinology; (United States)

OSTI ID:6296041

Puzas, J E; Brand, J S

Cells isolated from fetal rat calvaria were cultured in a (/sup 14/C)glycine-labeled collagen gel. This method of culture places the cells in an environment similar to that in the intact tissue and provides a means for assaying the release of collagenase from the cells. Degradation of the collagen matrix begins within the first 3 h of cell culture, the earliest time point at which samples were taken, and continues for at least 12 h. When cells were prepared from calvaria incubated for 30 min with parathyroid hormone (PTH), there was a marked decrease in the collagenase content of the freshly isolated cells, indicating that secretion of collagenase had been enhanced by PTH. Upon subsequent culture of control and PTH-pretreated cells, there was an increase in lysis of collagen gels surrounding the hormone-treated cells within 8 h. The increased collagenolysis was prevented by exposure of the cells to puromycin before and during culture, suggesting that collagenase synthesis also was stimulated by PTH.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6296041
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 104:2; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BONE CELLS
CELL CULTURES
CHEMICAL REACTIONS
COLLAGEN
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DECOMPOSITION
DRUGS
ENZYMES
EXPERIMENTAL DATA
FETUSES
GRAPHS
HORMONES
INFORMATION
ISOLATED VALUES
LYSIS
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PROTEINS
PUROMYCIN
RATS
RODENTS
SCLEROPROTEINS
SECRETION
SOMATIC CELLS
VERTEBRATES