Bone cell collagenase
Technical Report
·
OSTI ID:7245658
The enzyme necessary to initiate degradation of the organic collagen phase of bone was found in the culture fluids of resorbing bone. It is a true collagenase which is substrate specific, Ca/sup +/2 requiring, inhibited by serum factors and cleaves a tropocollagen molecule into characteristic macromolecular fragments. The enzyme was also demonstrated in an extract of freshly isolated fetal bone cells. In addition the secretory mechanism that is present in vivo was also present in the isolated cell preparation. In order to demonstrate an effect of parathyroid hormone (PTH) and other bone metabolic modifiers on the synthesis and secretion of collagenase a culture-assay system was developed. (auth)
- Research Organization:
- Rochester Univ., N.Y. (USA). School of Medicine and Dentistry
- OSTI ID:
- 7245658
- Report Number(s):
- UR-3490-884
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY
BONE TISSUES
CHEMICAL REACTIONS
COLLAGEN
CONNECTIVE TISSUE
DECOMPOSITION
ENZYMES
HORMONES
HYDROLASES
KINETICS
METABOLISM
MOLECULAR BIOLOGY
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PROTEINS
REACTION KINETICS
SCLEROPROTEINS
TISSUE CULTURES
TISSUE EXTRACTS
TISSUES
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BODY
BONE TISSUES
CHEMICAL REACTIONS
COLLAGEN
CONNECTIVE TISSUE
DECOMPOSITION
ENZYMES
HORMONES
HYDROLASES
KINETICS
METABOLISM
MOLECULAR BIOLOGY
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PROTEINS
REACTION KINETICS
SCLEROPROTEINS
TISSUE CULTURES
TISSUE EXTRACTS
TISSUES