Aerobic glycolysis in bone: lactate production and gradients in calvaria

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Am. J. Physiol.; (United States)

OSTI ID:5114216

Neuman, W F; Neuman, M W; Brommage, R

The experiments utilized specially designed Ussing chambers to measure lactate production and release by intact cranial bones in vitro. The net outflow of lactate was measured from both the inner and outer surfaces of cranial bones (18-day-old embryonic chicks and 2- to 3-day old rat pups were the donor sources). The net outward fluxes were surprisingly large: 0.5-1.0 ..mu..mol/cm/sup 2/.h; so large that the skeleton must be considered an important source of lactate in the overall carbohydrate economy of the intact animal. Lactate production was inhibited by lowered pH, external lactate, and disruption of the K/sup +/ gradient between bone and the external medium. Lactate production was increased by pretreatment with parathyroid hormone or an inhibitor of phosphorylation, carbonyl cyanide 3-chlorophenylhydrazone (CCCP). A good, positive correlation was observed between serum calcium in the rat pup and lactate production in vitro. Measurements of the lactate levels in the bone fluid showed that a real gradient exists, in concentrations 5 mM or more greater than those of the external medium.

• Research Organization: Univ. of Rochester Medical Center, NY
• OSTI ID: 5114216
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 234:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
ARYL RADICALS
BIOLOGICAL MATERIALS
BIRDS
BLOOD SERUM
BODY
BODY FLUIDS
BONE CELLS
BONE TISSUES
CALCIUM
CARBOHYDRATES
CARBONYL RADICALS
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHEMICAL REACTIONS
CHICKENS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
CYANIDES
DECOMPOSITION
ELEMENTS
FOWL
GLYCOLYSIS
HORMONES
HYDRAZONES
HYDROXY ACIDS
IONS
LACTIC ACID
MAMMALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARATHORMONE
PEPTIDE HORMONES
PH VALUE
PHENYL RADICALS
POTASSIUM IONS
RADICALS
RATS
RODENTS
SKELETON
SOMATIC CELLS
TISSUES
VERTEBRATES