Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor. delta. -chain diversity region and results in a previously unreported fusion transcript

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.6.2031· OSTI ID:5052079

Begley, C G; Aplan, P D; Davey, M P; Nakahara, K; Tchorz, K; Cohen, D I; Waldmann, T A; Kirsch, I R ^[1]; Kurtzberg, J; Hershfield, M S; Haynes, B F ^[2]

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1. National Institutes of Health, Bethesda, MD (USA)
2. Duke Univ. Medical Center, Durham, NC (USA)

The authors have studied a leukemic stem-cell line, DU.528, that is able to differentiate into myeloid and lymphoid cells. The leukemic cells have a translocation between chromosomes 1 and 14, t(1;14)(p33;q11), which they have molecularly cloned and sequenced. Initial screening used joining (J)-segment probes from the T-cell receptor (TCR) {alpha}- and {delta}-chain loci. In apparent concert with the translocation, a deletion has occurred between {delta}-chain diversity (D)-region genes D{sub {delta}1} and D{sub {delta}2}. The nature of the D{sub {delta}1}-D{sub {delta}2} deletional event implicates a lymphoid recombinase in the mechanism of the translocation. As a consequence of the translocation, an unusual fusion transcript was generated. Probes from chromosome 1 detected a previously unreported transcript in RNA from both the cell line and the patient. A chromosome 14 probe identified the same transcript, thus confirming a fusion transcript derived from both chromosomes 1 and 14. This translocation may identify a gene for which they propose the name SCL (stem-cell leukemia) that is important for hemopoietic development and oncogenesis and that has been disrupted or altered in this stem-cell line.

• OSTI ID: 5052079
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
RECEPTORS
TUMOR CELLS
CHROMOSOMAL ABERRATIONS
CELL DIFFERENTIATION
DNA HYBRIDIZATION
HETEROCHROMOSOMES
KARYOTYPE
LYMPHOCYTES
ONCOGENES
STEM CELLS
TRANSCRIPTION
TRITIUM COMPOUNDS
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
GENES
HYBRIDIZATION
HYDROGEN COMPOUNDS
LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
MUTATIONS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
550401* - Genetics- Tracer Techniques