t(8; 14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region
Recent molecular analyses of Burkitt lymphomas carrying the t(8;14) chromosome translocation have indicated that a dichotomy exists regarding the molecular mechanisms by which the translocations occur. Most sporadic Burkitt tumors carry translocations that apparently arise due to mistakes in the immunoglobulin isotype-switching process. In contrast, there is evidence that the translocations of most endemic Burkitt lymphomas occur as a consequence of aberrant V-D-J recombination of variable, diversity, and joining gene segments, catalyzed by the recombinase enzymes. This phenomenon was first noted in follicular lymphomas and chronic lymphocytic leukemias of the B-cell lineage and has been described in T-cell malignancies as well. In each of these cases, analysis of the nucleotide sequence at chromosome breakpoints demonstrated the involvement of immunoglobulin heavy chain J/sub H/ or T-cell-receptor ..cap alpha..-chain J..cap alpha.. gene segments in the translocation. The authors now have cloned and sequenced both the 8q- and 14q+ translocation breakpoints deriving from the t(8;14) translocation of the endemic Burkitt lymphoma line Daudi. The data show that the translocation resulted from a reciprocal exchange between the D/sub H/ region on chromosome 14 and sequences far 5' of the MYC protooncogene on chromosome 8. Features of the nucleotide sequences surrounding the breakpoint further implicate the V-D-J joining machinery in the genesis of chromosome translocation in endemic Burkitt lymphomas and, more generally, in other lymphoid malignancies as well.
- Research Organization:
- Wistar Institute, Philadelphia, PA (USA)
- OSTI ID:
- 7242026
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:19
- Country of Publication:
- United States
- Language:
- English
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CHROMOSOMAL ABERRATIONS
DNA SEQUENCING
DNA-CLONING
GENETIC MAPPING
LYMPHOMAS
GENETICS
ELECTROPHORESIS
GENE RECOMBINATION
IMMUNOGLOBULINS
MAN
PHOSPHORUS 32
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
GLOBULINS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
MUTATIONS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401* - Genetics- Tracer Techniques