Molecular resemblance of an AIDS-associated lymphoma and endemic Burkitt lymphomas: Implications for their pathogenesis

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.8907· OSTI ID:6906459

Haluska, F G; Russo, G; Croce, C M ^[1]; Kant, J ^[2]; Andreef, M ^[3]

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1. Fels Institute for Cancer Research and Molecular Biology, Philadelphia, PA (USA)
2. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)
3. Memorial Sloan Kettering Institute, New York, NY (USA)

Non-Hodgkin lymphoma is a common feature of AIDS. Approximately 30-40% of these tumors exhibit clinical features suggestive of endemic Burkitt lymphoma: they are aggressive malignancies that occur in association with Epstein-Barr virus infection, they arise in the setting of immunosuppression, and they carry t(8;14) translocations without detectable rearrangement of the MYC oncogene. To understand the molecular basis of these parallels, the authors analyzed a case of Epstein-Barr-positive AIDS-associated undifferentiated lymphoma. Southern blots show that the tumor exhibits immunoglobulin joining segment rearrangement but no rearrangement of the MYC oncogene. Cloning of the rearranged joining segment allowed the isolation of recombinant clones encompassing the translocation breakpoint, and sequencing of the translocation junction disclosed that the breakpoint is situated 7 base pairs from the chromosome 14 site involved in a previously described endemic Burkitt lymphoma translocation. Furthermore, the breakpoint is situated far from MYC on chromosome 8, a constant finding in endemic Burkitt lymphomas. That the molecular architecture of the translocation in this case is strikingly similar to previously analyzed translocations from endemic Burkitt lymphomas strongly suggests that common molecular mechanisms must be operative in the pathogenesis of these tumors.

• OSTI ID: 6906459
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:22; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DISEASES
DNA SEQUENCING
ENZYME IMMUNOASSAY
GENES
GENETICS
GLOBULINS
HEMIC DISEASES
HETEROCHROMOSOMES
IMMUNE SYSTEM DISEASES
IMMUNOASSAY
IMMUNOGLOBULINS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
LYMPHOMAS
MALES
MAMMALS
MAN
MEN
MUTATIONS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
PATHOGENESIS
PATIENTS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRAL DISEASES