Studies on a transplantable C57BL/6 mouse lymphoma. [X radiation]
Thesis/Dissertation
·
OSTI ID:5038644
A C57BL/6 mouse lymphoma was demonstrated to be of T cell origin by treating the lymphoma cells with anti-Thy 1.2 antisera in a complement-dependent cytotoxicity test. The lymphoma's growth pattern was described using flow microfluorometric determinations and spleen weight progression. C-type particles were identified in electron micrographs of the lymphoma. C57BL/6 mice were immunized against the lymphoma by injecting x-ray inactivated lymphoma cells into the mice. Protection of immunized mice against live lymphoma cells demonstrated tumor antigens on the lymphoma cells. The success of immunization was found to depend on: route of injection, antigen dosage, state of the antigen, number of injections and the vaccination-challenge interval. Attempts were made to passively transfer immunity from immunized C57BL/6 mice which had survived lymphoma challenge to non-treated, syngeneic mice. The route of immunization in the donors influenced the success of passively transferred immunity in the recipients. Serum from days 1 to 3 and days 11 to death (day 17) had an enhancing effect on lymphoma growth. However, sera from days 5 to 9 retarded lymphoma growth. The C57BL/6 lymphoma cells were injected into rabbits and other strains of mice to demonstrate tumor specificity. The lymphoma did not grow in rabbits and only grew in one mouse strain. This strain had the same major histocompatibility loci as C57BL/6 mice. Crosses were made between C57BL/6 mice and a resistant strain of mice (DBA/2 mice). The F/sub 1/ hybrids were found to be less susceptible to the lymphoma than the C57BL/6 strain. Sublethal x-irradiation of the F/sub 1/ mice decreased its ability to resist the C57BL/6 lymphoma. Immunization with x-ray inactivated C57BL/6 lymphoma cells increased survival after challenge with lymphoma in the F/sub 1/ mice.
- Research Organization:
- Texas Univ., Austin (USA)
- OSTI ID:
- 5038644
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
GROWTH
HYBRIDIZATION
IMMUNE REACTIONS
IMMUNE SERUMS
IONIZING RADIATIONS
LYMPHOMAS
MAMMALS
MICE
NEOPLASMS
RABBITS
RADIATION EFFECTS
RADIATIONS
RODENTS
TRANSPLANTS
TUMOR CELLS
VERTEBRATES
X RADIATION
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DISEASES
ELECTROMAGNETIC RADIATION
GROWTH
HYBRIDIZATION
IMMUNE REACTIONS
IMMUNE SERUMS
IONIZING RADIATIONS
LYMPHOMAS
MAMMALS
MICE
NEOPLASMS
RABBITS
RADIATION EFFECTS
RADIATIONS
RODENTS
TRANSPLANTS
TUMOR CELLS
VERTEBRATES
X RADIATION