Vaccination of adult and newborn mice of a resistant strain (C57BL/6J) against challenge with leukemias induced by Moloney murine leukemia virus

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Cancer Res.; (United States)

OSTI ID:5854607

Reif, A E

Adult or newborn C57BL/6J mice were immunized with isogenic Moloney strain MuLV-induced leukemia cells irradiated with 10,000 rads or treated with low concentrations of formalin. Groups of immunized and control mice were challenged with a range of doses of viable leukemia cells, and tumor deaths were recorded for 90 days after challenge. Then, the doses of challenge cells which produced 50% tumor deaths were calculated for immunized and control mice. The logarithm of their ratio quantified the degree of protection provided by immunization. For adult C57BL/6J mice, a single immunization with MuLV-induced leukemia cells was not effective; either cells plus Bacillus Calmette-Guerin or Corynebacterium parvum, or else two immunizations with irradiated leukemia cells were needed to produce statistically significant increases in the values of the doses of challenge cells which produced 50% tumor deaths. Cross-protection was obtained by immunization with other isogenic MuLV-induced leukemias, but not by immunization with isogenic carcinogen-induced tumors or with an isogenic spontaneous leukemia. For newborn mice, a single injection of irradiated leukemia cells provided 1.3 to 1.5 logs of protection, and admixture of B. Calmette-Guerin or C. parvum increased this protection to 2.4 to 2.7 logs. Since irradiated and frozen-thawed MuLV-induced leukemia cells contained viable MuLV, leukemia cells treated with 0.5 or 1.0% formalin were tested as an alternative. A single injection of formalin-treated isogenic leukemia cells admixed with C. parvum provided between 1.7 and 2.8 logs of protection. These results demonstrate that a single vaccination of newborn animals against a highly antigenic virally induced leukemia produces strong protection against a subsequent challenge with viable leukemia cells.

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 5854607
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 45:1; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
DISEASES
EXPERIMENTAL NEOPLASMS
HEMIC DISEASES
IMMUNITY
INOCULATION
IRRADIATION
LEUKEMIA
LEUKEMIA VIRUSES
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
RODENTS
VERTEBRATES
VIRUSES