Immunoprophylaxis and cytotoxic effector cells against EL 4 leukemia induced in syngeneic C57BL/6J mice by use of irradiated EL 4 cells

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · J. Natl. Cancer Inst., v. 55, no. 3, pp. 561-567

OSTI ID:4164171

Johnson, T S; Hudson, J L; Feldman, M E; Irvin, III, G L

Tumor-specific immunoprophylaxis was achieved in C57BL/6J mice against EL 4 leukosis cell challenge by sensitization of the syngeneic host with multiple ip injections of irradiated EL 4 cells. A minimal radiation dose was used to replication-block EL 4 cells before inoculation, as defined by dose-response analysis of irradiated EL 4 cells. Multiple ip injections of irradiated EL 4 cells stimulated development of significant, yet relatively low, levels of cytotoxic lymphoid activity (CLA) in lymphoid cells of the peritoneal exudate as measured by in vitro $sup 51$Cr-release cytotoxicity assays. The specific temporal and frequency dependencies of the inoculation regimen for achieving immunoprophylaxis indicated that, in addition to CLA, other, short-lived, immune processes were important in the tumor rejection. These observations showed the capacity of the C57BL/6J host for tumor-specific immune recognition and rejection of the syngeneic EL 4 leukemia. The tumor rejection could be elicited solely by inoculations of irradiated EL 4 cells and did not require exogenous amplifiers, such as immunoadjuvants, chemical modifiers, and/or allogeneic immune information transfer. (auth)

• Research Organization: Veterans Administration Hospital, Miami, FL
• NSA Number: NSA-33-003236
• OSTI ID: 4164171
• Journal Information: J. Natl. Cancer Inst., v. 55, no. 3, pp. 561-567, Journal Name: J. Natl. Cancer Inst., v. 55, no. 3, pp. 561-567; ISSN JNCIA
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*IMMUNE REACTIONS-- BIOLOGICAL RADIATION EFFECTS
*LEUKEMIA-- IMMUNE REACTIONS
*TUMOR CELLS-- BIOLOGICAL RADIATION EFFECTS
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
CHROMIUM 51
LINEAR ACCELERATORS
LYMPHOCYTES
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
NEOPLASMS
PHOTONS
TRACER TECHNIQUES