In vivo natural antitumor resistance against murine EL-4 lymphoma cells in lethally irradiated syngeneic C57Bl/6 mice

Journal Article · Sat Aug 01 04:00:00 EDT 1987 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(87)90195-X· OSTI ID:6121430

Iorio, A M; Neri, M; Bonmassar, E; Titti, F; Rossi, G B

Natural resistance has been detected in lethally irradiated C57Bl/6 (B6) mice inoculated intravenously with the ascites form of a syngeneic B6 leukemia. EL-4 cells were injected into lethally irradiated (800 R) B6 mice and tumor cell proliferation was evaluated by /sup 125/IUdR uptake in different organs 4 days after the challenge. Differential growth of lymphoma cells was observed when young mice were injected as compared with older mice and when mice were treated with agents known to interfere with natural resistance (e.g., poly(I:C), FLV-P, carrageenan, cyclophosphamide, high doses of irradiated cells). Similar results were obtained by measuring rapid clearance of /sup 125/IUdR-labeled EL-4 cells from lungs of intact B6 mice. In vivo cold competition studies, employing EL-4 and several other tumor lines of the same or different haplotype, showed that only EL-4 and RBL-5 cells were capable of inhibiting syngeneic resistance against EL-4 tumor. On the contrary, YAC-1 lymphoma cells, the most susceptible target to natural killer-mediated cytotoxicity in vitro, did not compete. These results suggest that EL-4 cells express membrane determinants not detectable on normal H-2b parental bone marrow cells and are susceptible to natural resistance against hemopoietic tumor cells in lethally irradiated syngeneic B6 mice.

• Research Organization: Univ. of Perugia, Italy
• OSTI ID: 6121430
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 108:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

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560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMALS
ASCITES TUMOR CELLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
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DAYS LIVING RADIOISOTOPES
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IMMUNITY
IN VIVO
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IODINE 125
IODINE ISOTOPES
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
LETHAL IRRADIATION
LEUKOCYTES
LUNG CLEARANCE
LYMPHOCYTES
LYMPHOMAS
MAMMALS
MATERIALS
MICE
MOSAICISM
NEOPLASMS
NUCLEI
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RADIATION CHIMERAS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES