Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

In vitro reactivity of splenic lymphocytes from normal and uv-irradiated mice against syngeneic uv-induced tumors. [Methylcholanthene]

Journal Article · · J. Immunol.; (United States)
OSTI ID:7110125
;
Skin tumors induced in mice by chronic ultraviolet (uv) irradiation are highly antigenic and are frequently immunologically rejected upon transplantation to normal syngeneic recipients. In this study we characterized this immune response with an in vitro microcytotoxicity test. Cytotoxic activity was present in the spleen cells of mice given a single injection of syngeneic uv-induced fibrosarcoma cells. After removal of adherent spleen cells, the remaining splenic lymphocytes were specifically cytotoxic for the immunizing tumor and showed no cross-reactivity with other syngeneic uv-induced or methylcholanthrene-induced tumors of similar histologic type. The level of cell-mediated reactivity against uv-induced tumors was quite high compared to that obtained with syngeneic tumors induced by methylcholanthrene, and the cytotoxicity was attributable to a population of theta antigen-bearing lymphocytes. With this in vitro test, we compared the response of normal mice, which reject a syngeneic tumor challenge, with that of uv-irradiated mice, in which the syngeneic uv-induced tumors grow progressively. After tumor cell inoculation, lymphocytes from the unirradiated (regressor) mice showed a high degree of cytotoxicity that reached a maximum level 8 days after injection. In contrast, no reactivity could be detected in the spleens of tumor-challenged uv-irradiated (progressor) mice.
Research Organization:
NCI Frederick Cancer Research Center, Frederick, MD
OSTI ID:
7110125
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 118:4; ISSN JOIMA
Country of Publication:
United States
Language:
English

Similar Records

Immune response to UV-induced tumors: mediation of progressor tumor rejection by natural killer cells
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5415513
Cell-mediated immune response to syngeneic uv induced tumors. I. The presence of tumor associated macrophages and their possible role in the in vitro generation of cytotoxic lymphocytes
Journal Article · Thu Nov 30 23:00:00 EST 1978 · Cell. Immunol.; (United States) · OSTI ID:6432515
Immune response to uv-induced tumors: transplantation immunity and lymphocyte populations exhibiting anti-tumor activity
Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984 · OSTI ID:7107316

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYL RADICALS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHOLANTHRENE
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
METHYL RADICALS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOINDUCTION
RODENTS
SKIN
SOMATIC CELLS
SPLEEN CELLS
TRANSPLANTS
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES