Protective immunization with B16 melanoma induces antibody response and not cytotoxic T cell response
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5386615
C57BL/6 mice immunized with three intraperitoneal injections of syngeneic, irradiated B16 melanoma cells, became resistant to B16 tumor challenge. Immunized mice had high levels of serum antibody against a membrane antigen of B16 cells. The B16 antigen recognized by the anti-B16 sera formed a major band of 90 KD in gel electrophoresis. The anti-B16 antibody was partially protective when mixed with B16 cells and injected into normal recipient mice. Surprisingly, B16 resistance mice were incapable of generating cytotoxic T cells (CTL) specific for the B16 tumor. Both spleen and lymph node cell populations from immunized mice did not generate B16-specific CTL. Allogeneic mice (DBA/2 or C3H) were also unable to generate B16-specific CTL: however, alloreactive CTL produced in these strains of mice by immunization with C57BL/6 lymphocytes, did kill B16 target cells. Interestingly, spleen cells from syngeneic mice immunized with B16 tumor produced 6-fold more interleukin-2 (IL-2) than normal spleen cells, in vitro. These data suggest that immunization with B16 tumor activates a helper subset of T cells (for antibody and IL-2 production) but not the effector CTL response.
- Research Organization:
- Univ. of Texas Medical Branch, Galveston
- OSTI ID:
- 5386615
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Helper T cells against tumor-associated antigens (TAA): preferential induction of helper T cell activities involved in anti-TAA cytotoxic T lymphocyte and antibody responses
Genetic control of cell-mediated lympholysis to trinitrophenyl (TNP)-modified murine syngeneic cells. Expression of Ir gene function at the cytotoxic precursor and helper cell
Rejection of B16 melanoma induced by expression of a transfected major histocompatibility complex class I gene
Journal Article
·
Mon Mar 31 23:00:00 EST 1986
· J. Immunol.; (United States)
·
OSTI ID:6776780
Genetic control of cell-mediated lympholysis to trinitrophenyl (TNP)-modified murine syngeneic cells. Expression of Ir gene function at the cytotoxic precursor and helper cell
Journal Article
·
Sat Feb 28 23:00:00 EST 1981
· J. Immunol.; (United States)
·
OSTI ID:5677519
Rejection of B16 melanoma induced by expression of a transfected major histocompatibility complex class I gene
Journal Article
·
Thu Mar 31 23:00:00 EST 1988
· Mol. Cell. Biol.; (United States)
·
OSTI ID:7192094
Related Subjects
550901 -- Pathology-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROPHORESIS
IMMUNITY
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
IRRADIATION
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MELANOMAS
MICE
NEOPLASMS
ORGANS
RODENTS
SOMATIC CELLS
SPLEEN
TUMOR CELLS
VERTEBRATES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROPHORESIS
IMMUNITY
INJECTION
INTAKE
INTRAPERITONEAL INJECTION
IRRADIATION
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MELANOMAS
MICE
NEOPLASMS
ORGANS
RODENTS
SOMATIC CELLS
SPLEEN
TUMOR CELLS
VERTEBRATES