Hyaluronate synthesis by synovial villi in organ culture. [Dogs]

Journal Article · Wed Jun 01 04:00:00 EDT 1983 · Arthritis Rheum.; (United States)

DOI:https://doi.org/10.1002/art.1780260610· OSTI ID:5024563

Myers, S L; Christine, T A

Individual canine synovial villi were used to establish short-term synovial organ cultures. These villi incorporated /sup 3/H-glucosamine into highly-polymerized /sup 3/H-hyaluronic acid (/sup 3/H-HA), which was the only /sup 3/H-glycosaminoglycan identified in the culture medium. Some /sup 3/H-HA, and larger amounts of other /sup 3/H-glycosaminoglycans, were recovered from cultured tissues. Culture medium /sup 3/H-HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less /sup 3/H-HA per mm2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis
• OSTI ID: 5024563
• Journal Information: Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 26:6; ISSN ARHEA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
BIOCHEMISTRY
BIOSYNTHESIS
BODY
BONE JOINTS
CARBOHYDRATES
CHEMISTRY
DOGS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
HYALURONIC ACID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
SACCHARIDES
SKELETON
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES