Hyaluronic acid production by irradiated human synovial fibroblasts

Journal Article · Tue Mar 01 04:00:00 EST 1977 · Arthritis Rheum.; (United States)

DOI:https://doi.org/10.1002/art.1780200210· OSTI ID:7219779

Yaron, M; Yaron, I; Levita, M; Herzberg, M

Radioactive particles as well as x irradiation from an external source has been used in the treatment of rheumatoid arthritis, osteoarthritis, and ankylosing spondylitis. In order to clarify effects of ionizing irradiation on synovial cells, radioactive gold (/sup 198/Au) and yttrium (/sup 90/Y) were added to fibroblast cultures derived from human synovial membranes. Other cultures were irradiated by a Picker x-ray machine. Fibroblast growth and hyaluronic acid production were measured. Radioactive gold and yttrium particles induced a significant increase of hyaluronic acid synthesis rate (pg/cell/day) and inhibited fibroblast growth. Fibroblasts continued to overproduce hyaluronic acid and to show growth inhibition 3 weeks after irradiation with radioactive gold. Hydrocortisone inhibited hyaluronic acid overproduction induced by radioactive gold. Overproduction of hyaluronic acid induced by the x-ray machine was inhibited by hydrocortisone, actinomycin-D, and cycloheximide. Fibroblasts derived from normal and rheumatoid patients responded similarly to ionizing irradiation.

• Research Organization: Rokach (Hadassah) and Ichilov Medical Center, Tel Aviv, Israel
• OSTI ID: 7219779
• Journal Information: Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 20:2; ISSN ARHEA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560122 -- Radiation Effects on Cells-- Internal Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINOMYCIN
ADRENAL HORMONES
AMINES
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BONE JOINTS
CARBOHYDRATES
CELL CULTURES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
FUNGICIDES
GLUCOCORTICOIDS
GOLD 198
GOLD ISOTOPES
HEAVY NUCLEI
HORMONES
HOURS LIVING RADIOISOTOPES
HYALURONIC ACID
HYDROCORTISONE
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
MAMMALS
MAN
MUCOPOLYSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
POLYSACCHARIDES
PREGNANES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
SACCHARIDES
SKELETON
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
X RADIATION
YTTRIUM 90
YTTRIUM ISOTOPES