Extracellular depolymerization of hyaluronic acid in cultured human skin fibroblasts

Journal Article · Mon Oct 15 04:00:00 EDT 1990 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/S0006-291X(05)80174-3· OSTI ID:6323901

Nakamura, T; Takagaki, K; Kubo, K; Morikawa, A; Tamura, S; Endo, M ^[1]

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1. Hirosaki Univ. School of Medicine (Japan)

The chain length of ({sup 3}H)hyaluronic acid synthesized by cultivating human skin fibroblasts in the presence of ({sup 3}H)glucosamine was investigated. ({sup 3}H)Hyaluronic acid obtained from the matrix fraction was excluded from a Sepharose CL-2B column irrespective of the incubation period, whereas that from the medium was depolymerized into a constant chain length (Mr = 40,000). The reducing and non-reducing terminals of the depolymerized hyaluronic acid were N-acetylglucosamine and glucuronic acid, respectively. Prolonged incubation produced no oligosaccharides as shown by examination of hyaluronidase digests, suggesting the presence of a novel endo-beta-N-acetylglucosaminidase in cultured human skin fibroblasts.

• OSTI ID: 6323901
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 172:1; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
BODY
CARBOHYDRATES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ENZYMES
FIBROBLASTS
GLUCOSAMINE
GLYCOSYL HYDROLASES
HEXOSAMINES
HEXOSES
HYALURONIC ACID
HYDROGEN COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
MAMMALS
MAN
METABOLISM
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PRIMATES
SACCHARIDES
SEPARATION PROCESSES
SKIN
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES