Manganese peroxidase mRNA and enzyme activity levels during bioremediation of polycyclic aromatic hydrocarbon-contaminated soil with Phanerochaete chrysosporium

Journal Article · Mon Jul 01 04:00:00 EDT 1996 · Applied and Environmental Microbiology

OSTI ID:486430

Bogan, B W ^[1]; Schoenike, B; Lamar, R T; Cullen, D ^[2]

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1. Univ. of Wisconsin, Madison, WI (United States)
2. Forest Service Forest Products Lab., Madison, WI (United States)

mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of three manganese peroxidase (MnP) genes during removal of polycyclic aromatic hydrocarbons from cultures of Phanerochaete chrysosporium grown in presterilized soil. Periods of high mnp transcript levels and extractable MnP enzyme activity were temporally correlated, although separated by a short (1- to 2-day) lag period. This time frame also coincided with maximal rates of fluorene oxidation and chrysene disappearance in soil cultures, supporting the hypothesis that high ionization potential polycyclic aromatic hydrocarbons are oxidized in soil via MnP-dependent mechanisms. The patterns of transcript abundance over time in soil-grown P. chrysosporium were similar for all three of the mnp mRNAs studied, indicating that transcription of this gene family may be coordinately regulated under these growth conditions. 47 refs., 6 figs., 1 tab.

• DOE Contract Number: FG02-87ER13712
• OSTI ID: 486430
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 7 Vol. 62; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
CONTAMINATION
ECOLOGICAL CONCENTRATION
ENZYME ACTIVITY
MANGANESE COMPOUNDS
MESSENGER-RNA
PHANEROCHAETE
POLYCYCLIC AROMATIC HYDROCARBONS
REMEDIAL ACTION
SOILS