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Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood

Journal Article · · Applied and Environmental Microbiology
OSTI ID:658989
 [1]; ;  [2];  [2]
  1. Univ. of Stellenbosch (South Africa). Dept. of Microbiology
  2. Forest Products Lab., Madison, WI (United States). Inst. of Microbial and Biochemical Technology
+ Show Author Affiliations
Lignin depolymerization is catalyzed by extracellular enzymes of white rot basidiomycetes such as Phanerochaete chrysosporium. Major components of this system include lignin peroxidases, manganese-dependent lignin peroxidases, and a peroxide-generating enzyme, glyoxal oxidase. Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.
Sponsoring Organization:
USDOE, Washington, DC (United States)
DOE Contract Number:
FG02-87ER13712
OSTI ID:
658989
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 9 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
Country of Publication:
United States
Language:
English

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Related Subjects

09 BIOMASS FUELS
DEPOLYMERIZATION
GENE REGULATION
GENES
LIGNIN
PEROXIDASES
PHANEROCHAETE
RNA
WOOD