Expression of Phanerochaete chrysosporium genes encoding lignin peroxidases, manganese peroxidases, and glyoxal oxidase in wood

Journal Article · Tue Sep 01 00:00:00 EDT 1998 · Applied and Environmental Microbiology

OSTI ID:658989

Janse, B J.H. ^[1]; Gaskell, J; Cullen, D ^[2]; Akhtar, M ^[2]

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1. Univ. of Stellenbosch (South Africa). Dept. of Microbiology
2. Forest Products Lab., Madison, WI (United States). Inst. of Microbial and Biochemical Technology

Lignin depolymerization is catalyzed by extracellular enzymes of white rot basidiomycetes such as Phanerochaete chrysosporium. Major components of this system include lignin peroxidases, manganese-dependent lignin peroxidases, and a peroxide-generating enzyme, glyoxal oxidase. Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.

• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-87ER13712
• OSTI ID: 658989
• Journal Information: Applied and Environmental Microbiology, Vol. 64, Issue 9; Other Information: PBD: Sep 1998
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
GENE REGULATION
LIGNIN
WOOD
PEROXIDASES
GENES
PHANEROCHAETE
RNA
DEPOLYMERIZATION