Overproduction of lignin-degrading enzymes by an isolate of Phanerochaete chrysosporium

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5755329

Orth, A B; Denny, M; Tien, Ming ^[1]

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1. Pennsylvania State Univ., University Park (United States)

Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. PSBL-1 is a mutant of this organism that generates the ligninolytic system under nonlimiting conditions during primary metabolism. Lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities for PSBL-1 under nonlimiting conditions were 4- to 10-fold higher than those of the wild type (WT) under nitrogen-limiting conditions. PSBL-1 was still in the log phase of growth while secreting the enzymes, whereas the WT had ceased to grow by this time. As in the WT, manganese(II) increased manganese peroxidase activity in the mutant. However, manganese also caused in increase in lignin peroxidase and glyoxal oxidase activities in PSBL-1. Addition of veratryl alcohol to the culture medium stimulated lignin peroxidase activity, inhibited glyoxal oxidase activity, and had little effect on manganese peroxidase activity in PSBL-1, as in the WT. Fast protein liquid chromatography (FPLC) analysis shows production of larger amounts of isozyme H2 in PSBL-1 than in the WT. These properties make PSBL-1 very useful for isolation of large amounts of all ligninolytic enzymes for biochemical study, and they open the possibility of scale-up production for practical use.

• DOE Contract Number: FG02-87ER13690
• OSTI ID: 5755329
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:9; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FUNGI
ENZYME ACTIVITY
LIGNIN
BIODEGRADATION
GLYOXAL
MANGANESE
MUTANTS
OXIDOREDUCTASES
PEROXIDASES
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
ENZYMES
METALS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
TRANSITION ELEMENTS
550200* - Biochemistry