Manganese peroxidase gene transcription in Phanerochaete chrysosporium: Activation by manganese

Journal Article · Mon Jul 01 04:00:00 EDT 1991 · Journal of Bacteriology; (United States)

OSTI ID:5699467

Brown, J A

The expression of manganese peroxidase in nitrogen-limited cultures of Phanerochaete chrysosporium is dependent on Mn, and initial work suggested that Mn regulates transcription of the mnp gene. In this study, using Northern (RNA) blot analysis of kinetic, dose-response, and inhibitor experiments, the authors demonstrate unequivocally that Mn regulates mnp gene transcription. The amount of mnp mRNA in cells of 4-day-old nitrogen-limited cultures is a direct function of the concentration of Mn in the culture medium up to a maximum of 180 {mu}M. Addition of Mn to nitrogen-limited Mn-deficient secondary metabolic (4-, 5-, and 6-day-old) cultures results in the appearance of mnp mRNA within 40 min. The appearance of this message is completely inhibited by the RNA synthesis inhibitor dactinomycin but not by the protein synthesis inhibitor cycloheximide. Furthermore, the amount of mnp mRNA produced is a direct function of the concentration of added Mn. In contrast, addition of Mn to low-nitrogen Mn-deficient 2- or 3-day-old cultures does not result in the appearance of mnp mRNA. Manganese peroxidase protein is detected by specific immunoprecipitation of the in vitro translation products of poly(A) RNA isolated from Mn-supplemented (but nor from Mn-deficient) cells. All of these results demonstrate that Mn, the substrate for the enzyme, regulates mnp gene transcription via a growth-stage-specific and concentration-dependent mechanism.

• DOE Contract Number: FG06-87ER13715
• OSTI ID: 5699467
• Journal Information: Journal of Bacteriology; (United States), Journal Name: Journal of Bacteriology; (United States) Vol. 173:13; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
ENZYMES
FUNGI
GENES
INHIBITION
KINETICS
MANGANESE
MESSENGER-RNA
METALS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PLANTS
PROTEINS
REACTION KINETICS
RNA
TRANSCRIPTION
TRANSITION ELEMENTS