Regulation of manganese peroxidase gene transcription by hydrogen peroxide, chemical stress, and molecular oxygen

Journal Article · Sun Jan 01 04:00:00 EST 1995 · Applied and Environmental Microbiology

OSTI ID:75824

Li, D; Alic, M; Brown, J A; Gold, M H ^[1]

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1. Oregon Graduate Institute of Science and Technology, Portland, OR (United States)

The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of the lignin-degrading fungus Phanerochaete chrysosporium is regulated at the level of gene transcription by H{sub 2}O{sub 2} and various chemicals, including ethanol, sodium arsenite, and 2,4-dichlorophenol, as well as by Mn(II) and heat shock. Northern (RNA) blot analysis demonstrates that the addition of 1.0 mM H{sub 2}O{sub 2} to 5-day-old cultures grown in the absence of Mn results in the appearance of mnp mRNA within 15 min. Higher levels of mnp mRNA are obtained with simultaneous induction by Mn and H{sub 2}O{sub 2} than with H{sub 2}O{sub 2} alone. Although neither MnP activity nor associated protein is detectable in H{sub 2}O{sub 2}-induced cultures grown in the absence of Mn, simultaneous induction with Mn and H{sub 2}O{sub 2} results in a 1.6-fold increase in MnP activity compared with the MnP activity resulting resulting from Mn induction alone. In the presence of Mn, purging of low-nitrogen cultures with 100% O{sub 2}, in contrast to incubation under air, results in an increase in the accumulation of mnp mRNA and a 13-fold increase in MnP activity on day 5. However, in contrast to the effects of H{sub 2}O{sub 2} and heat shock, O{sub 2} purging of Mn-deficient cultures results in negligible accumulation of mnp mRNA. 48 refs., 6 figs.

• DOE Contract Number: FG06-92ER20093
• OSTI ID: 75824
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 1 Vol. 61; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOCHEMISTRY
BIOLOGICAL STRESS
ECOLOGICAL CONCENTRATION
GENES
HYDROGEN PEROXIDE
OXYGEN
PEROXIDASES
PHANEROCHAETE
TRANSCRIPTION