Expression of lip genes during growth in soil and oxidation of anthracene by Phanerochaete chrysosporium

Journal Article · Tue Oct 01 04:00:00 EDT 1996 · Applied and Environmental Microbiology

OSTI ID:508350

Bogan, B W ^[1]; Schoenike, B; Lamar, R T; Cullen, D ^[2]

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1. Univ. of Wisconsin, Madison, WI (United States)
2. Inst. for Microbial and Biochemical Technology, Madison, WI (United States)

mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of the 10 known lignin peroxidase (lip) genes in anthracene-transforming soil cultures of Phanerochaete chrysosporium. Levels of extractable lipA transcript and protein (LiP H8) were well correlated, although they were separated by a 2-day lag period. The patterns of transcript abundance over time in soil-grown P. chrysosporium varied among the nine lip mRNAs detected; comparison with lip gene expression under different liquid culture conditions suggested an early phase of carbon limitation for the cultures as a whole, which was followed by a transition to nitrogen starvation. Anthracene transformation occurred through-out the 25-day course of the experiment and, therefore, likely involves mechanisms distinct from those involved in oxidation of non-LiP substrate polycyclic aromatic hydrocarbons. 54 refs., 6 figs.

• DOE Contract Number: FG02-87ER13712
• OSTI ID: 508350
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 10 Vol. 62; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANTHRACENE
BIODEGRADATION
BIOLOGICAL PATHWAYS
GENES
PHANEROCHAETE