STUDIES ON THE COMBINED EFFECT OF RADIATION AND VARIOUS CHEMICALS ON MITOTIC CELLS. XIV. EFFECT OF THE COMBINED USE OF X-RAY AND ACRIFLAVINE
The effect on sarcoma cells of acriflavine, alone and combined with radiation, was tested. The material used was MTK sarcoma 111 of rats. An aqueous solution of acriflavine was injected intraperitoneally, and the frequency of mitosis, the percentage of each mitetic phase, and the frequency of abnormal chromosomes in the metaphase and anaphase were determined. The frequency of mitosis diminished rapidly after the administration of acriflavine, being lowest 3 to 6 hr after injection. Then the number of mitotic cells gradually increased, but it was not restored to the pre-treatment condition even 48 hr after injection. The decrease in mitoses was proportional to the amount of acriflavine injected, rarging from 0.1 mg/100 g to 2.5 mg/ 100 g. Abnormal chromosomes with stickiness and condensation were found in the metaphase, while in the anaphase chromosome- bridges induced by stickiness were definitely increased. When combined with x ray the number of mitoses in sarcoma cells was even fewer. When small amounts of acriflavine (up to 0.005 mg/100 g) were administered before or after irradiation, mitosis of sarcoma cells was depressed, although no recognizable effect occurred when such small amounts were used without x irradiation. (Abstr. Japan Med., 1: No. 10, 1961)
- Research Organization:
- Hokkaido Univ. School of Medicine, Sapporo
- NSA Number:
- NSA-15-027446
- OSTI ID:
- 4843772
- Journal Information:
- Nippon Igaku Hoshasen Gakkai Zasshi (Japan), Journal Name: Nippon Igaku Hoshasen Gakkai Zasshi (Japan) Vol. Vol: 19; ISSN NHGZA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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