STUDIES ON THE COMBINED EFFECT OF RADIATION AND VARIOUS CHEMICALS ON MITOTIC CELLS. XIII. EFFECT OF COMBINED USE OF X-RAYS AND SUCCINIC ACID
Succinic acid alone and combined with x rays and malonic acid was tested for its effect on sarcoma cells. The material used was the MTK sarcoma 111 of rat, 3 to 4 days after transplantation. An aqueous solution of succinic acid was injected intraperitoneally in rats, and the frequency of mitosis, the percentage of each mitotic phase, and the frequency of abnormal chromosomes in the metaphase were determined. Succinic acid caused a great increase in the number of mitotic cells. Mitotic cells in the prophase and metaphase increased in parallel with the mitotic index, and those in the anaphase and telophase appeared with some lapse of time. The increase in mitotic cells was almost the same with the succinic acid dose range of 10 mg to 68 mg/ 100 g. In the metaphase, abnormal chromosomes showing signs of stickiness or coalescence were observed. Injection of succinic acid before x irradiation counteracted the mitosis-depressing effect of irradiation. Injection of succinic acid after irradiation restored the mitosis to a marked degree if it were given at least 3 hours after the irradiation, otherwise it was ineffective. The action of succinic acid was antagonized by malonic acid temporarily, but then succinic acid overcame the effect of the latter and accelerated mitosis. In the combined use of succinic acid with malonic acid and x rays, succinic acid could not restore mitosis. (Abstr. Japan Med., No. 8, 1961)
- Research Organization:
- Hokkaido Univ., Sapporo
- NSA Number:
- NSA-15-022092
- OSTI ID:
- 4045188
- Journal Information:
- Nippon Igaku Hoshasen Gakkai Zasshi (Japan), Journal Name: Nippon Igaku Hoshasen Gakkai Zasshi (Japan) Vol. Vol: 19; ISSN NHGZA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
STUDIES ON THE COMBINED EFFECTS OF RADIATION AND VARIOUS CHEMICALS ON THE MITOTIC CELLS. XXV. EFFECT OF COMBINED USE OF X-RAYS AND MALEIC ACID
STUDIES ON THE COMBINED EFFECT OF RADIATION AND VARIOUS CHEMICALS ON MITOTIC CELLS. XII. EFFECT OF COMBINED USE OF X-RAY AND ZnSO$sub 4$