STUDIES ON THE COMBINED EFFECT OF IRRADIATION AND VARIOUS CHEMICALS ON MITOTIC CELLS. XVII. EFFECTS OF COMBINED USE OF MITOMYCIN C AND X-RAYS
The MTK sarcoma III of a rat, 3 to 4 days after transplantation was adopted as material for this study. Mitomycin C was injected into the peritoneal cavity of the rat. The effect was judged by the frequency of mitosis, the difference of each mitotic phase in number, the frequency of abnormalities of chromosomes in the metaphase and the anaphase, and the life span of the tumor- bearing rat. Mitomycin C in the amount of 0.001 to 0.1 mg/100 g caused a decrease of mitotic cells at 3 hr after its injection. Mitotic cells became fewest at 6 hr after injection, and after that they gradually increased in number. Rapid decreases in the number of mitotic cells were caused by 0.3 mg/100 g of the drug; all the cells disappeared at 48 hr after injection. The mitotic cells in each phase decreased in proportion to the mitotic index. Mitomycin C influenced both resting and dividing tumor cells. In the resting cells, vacuoles were formed in the cytoplasm following the change which occurred in the nucleus, and finally the cells were destroyed. In the dividing cells, stickiness or condensation of chromosomes was noticed; then, such abnormalities as breakage of chromosomes, formation of chromosomal bridges, or coalescences appeared. Injection of 0.01 mg/100 g of mitomycin C right after or 1 hr after x irradiation showed a much stronger effect in decreasing the mitotic cells of the sarcoma than either radiation or drugs alone. The combined effect in decreasing the mitotic cells of the sarcoma was increased further when injection of 0.01 mg/100 g mitomycin C was carried out before irradiation. Even a very small amount of mitomycin C, 0.001 mg/100 g, increased the effect in the combined use. The combined use of mitomycin C with x rays brought about an increase in the life of a tumor-bearing rat; 0.05 mg to 0.3 mg/100 g of the drug depressed the tumor almost completely, 0.01 mg/100 g also prolonged the life of the tumor-bearing rat, though the effect was less than in the former case. (Absts. Japan. Med., 1: No. 7, 1960.)
- Research Organization:
- Hokkaido Univ., Sapporo
- NSA Number:
- NSA-15-022109
- OSTI ID:
- 4068339
- Journal Information:
- Nippon Igaku Hoshasen Gakkai Zasshi (Japan), Journal Name: Nippon Igaku Hoshasen Gakkai Zasshi (Japan) Vol. Vol: 19; ISSN NHGZA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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