BONE MARROW TREATMENT OF MICE LETHALLY IRRADIATED WITH GAMMA-RAYS UNDER HIGH DOSE RATE. IV. SUPPLEMENTARY STUDIES

Journal Article · Thu Mar 01 04:00:00 EST 1962 · Bull. Inst. Chem. Research, Kyoto Univ.

OSTI ID:4830773

Yamagishi, M; Adachi, K; Shirakawa, S; Yamaba, M; Uchino, H

Mlce were lrradlated with gamma rays under hlgh dose rate (42 r per second) and then treated with lsologous, homologous, and heterologous bone marrow under varlous experimental condltions. By lncreasing the dose of lsologous bone marrow from lO x lO/sup 6/to 20 x l0/sup 6/ nucleated cells, a falrly good survlval rate was obtalned even when lsologous bone marrow (lBM) was lnjected as late as 6 days after lrradlatlon. Even with this dose lBM injectlon at 7 days was not effectlve ln obtalnlng survlvlng mlce. The IBM dose as small as O.l x l0/ sup 6/nucleated cells was effective ln obtalning falrly good survival rate when IBM was given immediately after lrradlation. However, larger IBM dose is desirable because of quicker regeneratlon of hematopoletlc organs ln mlce recelvlng a larger dose. The qulck recovery of hematopoletlc organs ln mlce lethally gamma lrradlated and treated with IBM was proved chemically by the early lncorporatlon of formate-C/sup 14/ into the bone marrow and spleen after lrradlatlon. Iron metabollsm in irradiated and lBM treated mlce was also studled. The delayed treatment wlth hontologous bone marrow (HBM) from one to four days post-irradiatlon seemed effectlve ln talnlng a good lnitlal l4 day survlval rate. Heterologous rat bone marrow (RBM) has so far been lneffectlve even b changlng experimental condltlons variously. When hom ogous donor mice were glven AET foliowed by 500 r (or100 r) lrradlation, nelther lnitlal nor subsequent survlval of lethally irradlated recipient was good. When reciplent ce were given AET followed by an otherwise supralethal ose of lrradlation (l500 r) good lnitial survival was ob lned. Bone marrow dose dld not seem to modlfy radlati n damage to tlssues other than those of the hematopolet c system as shown by retarded growth and reduced life an. Furthermore, evidences of late radiation effect suc as nephrosclerosls and calclflcation of myocardlum were observed ln gamma lrradiated and IBM treated mlce. (auth)

• Research Organization: Kyoto Univ.
• NSA Number: NSA-16-018799
• OSTI ID: 4830773
• Journal Information: Bull. Inst. Chem. Research, Kyoto Univ., Journal Name: Bull. Inst. Chem. Research, Kyoto Univ. Vol. Vol: 40
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BLOOD FORMATION
BONE MARROW
CARBON 14
GAMMA RADIATION
HEART
IRON
KIDNEYS
LETHAL DOSE
LIFETIME
METABOLISM
MICE
RADIATION DOSES
RADIATION INJURIES
RECOVERY
REGENERATION
TISSUES
TRACER TECHNIQUES
TRANSPLANTS