BONE MARROW TRANSPLANTATION STUDIES IN MICE LETHALLY IRRADIATED UNDER HIGH DOSE RATE (in Japanese)
When dd/s mice were exposed to 900-r, whole-bod Co/sup 60/ gamma radiation (42 r/sec) and transplanted with isologous bone marrow, a 40 to 100% 30- day survival was noted. Rematologic and histologic studies as well as those using radioisotope tracers revealed that the high survival rate was due to early and rapid recovery of hematopoietic function. Usually 10 million nucleated bone marrow cells were transplanted. When the nucleated cell number was increased to 20 million, isologous marrow transplantation even 8 days after irradiation still gave a survival rate >30%. Decreasing the amounts down to 100,000 still gave approximates 45% 30-day survival. Homologous bone marrow transplantation after lethal irradiation also gave good (30 to 70%) 14-day survival, followed by rapid increase of death rate. Histologic study revealed that recovery of cells of erythrocytic and myelocytic series occurred early, but that of lymphatic tissue was retarded. Recovery of lymphatic tissue became apparent only after 40 to 50 days postirradiation. Homologous marrow transplantation 1 or 2 days after irradiation seemed better in obtaining good 14-day survival than transplantation immediately after irradiation. Increasing the bone marrow dose up to 20 million nucleated cells was not effective in obtaining good initial survival. Transplantation studies of chimeric bone marrow showed the possible existence of homologous marrow in host mice both at 20 days and 8 1/2 months after transplantation. Successful transplantation of heterologous (rat) bone marrow was not obtained. When aminoethylisothiuronium was given to the host followed by otherwise supralethal (1500-r) irradiation, successful transplantation of homologous bone marrow seemed to be facilitated. Its use in bone marrow donors, combined with irradiation, may be of value, it is suggested, to prevent secondary disease in view of reports that AET protects mainly nonantibody-forming cells. Delayed effecte of radiation injury (shortening of life span, retarded body wt increase, and increased incidence of nephrosclerosis and focal myocardial calcification) were seen in the lethally irradiated mice treated with isologous bone marrow. (BBB)
- Research Organization:
- Kyoto Univ.
- NSA Number:
- NSA-17-038894
- OSTI ID:
- 4657484
- Journal Information:
- Nippon Ketsueki Gakkai Zasshi (Japan), Journal Name: Nippon Ketsueki Gakkai Zasshi (Japan) Vol. Vol: 24; ISSN NKGZA
- Country of Publication:
- Country unknown/Code not available
- Language:
- Japanese
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