Response of Neoplastic Cells in Vitro to Ionizing Radiation

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Annals of the New York Academy of Sciences

DOI:https://doi.org/10.1111/j.1749-6632.1961.tb50077.x· OSTI ID:4822159

Goldfeder, Anna

A brief historical survey of radiation effects on tumor cells in tissue culture is presented. The radiosensitivity of various types of normal and neoplastic cells was studied, and cytological abnormalities in the irradiated cells, such as chromatin bridges, double anaphases in the same cell, tripolar and tetrapolar mitoses, multinucleated giant cells, nuclear and cytoplasmic vacuolization, and liquefaction of the culture medium were noted. Doses of x rays ranging from 80,000 r to 150,000 r were required for total inhibition of growth of the tissue explanis in the cultures. Small doses permitted recovery. Evidence for a selective response to irradiation between various types of tissue cells, normal and malignant, is presented. Studies on a subcellular level by the aid of electronmicroscopic and microanalytic techniques revealed that the mitochondria of the more radiosensitive tumor are fewer in number and inferior in quality as compared to that of the more radioresistant tumor. The possible role these mitochondrial differences may play in cellular radiosensitivity is discussed.

• Research Organization: City of New York Dept. of Hospitals; and New York Univ., New York
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-026753
• OSTI ID: 4822159
• Journal Information: Annals of the New York Academy of Sciences, Journal Name: Annals of the New York Academy of Sciences Journal Issue: 2 Vol. 95; ISSN 0077-8923
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CANCER
CYTOLOGY
ELECTRON MICROSCOPY
MITOCHONDRIA
MITOSIS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RECOVERY
TUMORS
X RADIATION