THE EFFECT OF IONIZING RADIATION ON STRAIN L MONOLAYERS. I. CYTOLOGICAL ABERRATIONS AND CELL MULTIPLICATION

Journal Article · Fri Feb 01 04:00:00 EST 1963 · Life Sciences (U.S.) Divided into Parts I and II

DOI:https://doi.org/10.1016/0024-3205(63)90019-5· OSTI ID:4714630

Nardone, R M

A study was made of cytological aberrations caused by exposure of cells to 500, 1000, and 1500-r doses of Co/sup 60/ gamma rays at a rate of 1332 r/hr. The changes include cytoplasmic vacuolization, nuclear blistering, amitotic division, and giant-cell formation. An analysis of the effect of dose and postirradiation time on the mitotic index, and on the frequency of multinucleate cells and those with blistered nuclei, showed that the full impact of such high doses of radiation is not immediately manifested. The incidence of abnormal nuclei and the inhibition of mitosis are related to dose and time after irradiation. The irradiated cultures had a higher frequency of multinucleate cells than was found in nonirradiated cultures. However, the aberration could not be correlated with dose. The most evident cytoplasmic modifications were nuclear blistering and giant cell formation. The effects of 100- to 3000-r doses on growth of such populatlons were also studied. Only the 3000-r dose prevented an increase in cell number; all other doses resulted in a temporary cessation of population growth. The slopes of the growth curves of the 100to 1000-r cultures were very similar despite the accumulation of gross cytological abnormalities in the cultures irradiated with 500 and 100 r. Despite such gross nuclear abnormalities as blistering in some of its members, the 500-r population was able to increase at a rate close to that of the nonirradiated population, although the growth rate of cultures irradiated with more than 500 r was proportionately depressed. The differences are discussed. (TCO)

• Research Organization: Catholic Univ. of America, Washington, D.C.
• NSA Number: NSA-17-024949
• OSTI ID: 4714630
• Journal Information: Life Sciences (U.S.) Divided into Parts I and II, Journal Name: Life Sciences (U.S.) Divided into Parts I and II Vol. Vol: No. 2; ISSN LIFSA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
COBALT 60
CYTOLOGY
GAMMA RADIATION
GIANT CELLS
MITOSIS
POPULATIONS
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES