RADIATION STUDIES ON MAMMALIAN CELLS GROWN IN A CHEMICALLY DEFINED MEDIUM

Technical Report · Tue Jan 01 04:00:00 EST 1963

OSTI ID:4712698

Nardone, R M

Studies were made on the effects of ionizing radiation on the multiplication, size, gross cytology, protein and nucleic acid synthesis, and respiration of strain L (mouse fibroblast). Cultures irradiated with 100, 300, and 500 r, after a lag phase, manifest growth curves with slopes similar to those of nonirradiated cultures. Cultures irradiated with 1000 to 2000 r increase the lag phase and depress the growth curve slopes. Doses above 2500 r result in complete mitotic arrest. Doses of 500 to 1500 r resulted in nuclear blistering and gigantism, the frequency increasing with time after irradiation. In addition to cell muitiplication, growth in terms of culture volume (mass) and average cell size was studied. Forty-eight hours after irradiation the cell numbers in 500-r- exposed popuiations were 45% below that of controls while the cuiture volume was reduced 28% and the average cell size was increased 12%. Extracellular protein synthesis was more sensitive than intracellular synthesis, with inhibition in both experiments becoming more pronounced each day. Inhibition of RNA synthesis was dose and time correlated. RNA synthesis inhibition was much more sensitive than DNA synthesis in the same cells. Because of radioinduced gigantism, irradiated cells had much more protein and DNA per cell than those from nonirradiated cultures but the RNA content of giant cells was equal to that of normal cells. Warburg studies of endogenous respiration indicate that respiration inhibition is correiated with dose and duration of the post- irradiation period. (P.C.H.)

• Research Organization: Catholic Univ. of America, Washington, D.C.
• NSA Number: NSA-17-023092
• OSTI ID: 4712698
• Report Number(s): TID-18625
• Country of Publication: United States
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
CONTROL
CYTOLOGY
DNA
IONS
METABOLISM
MITOSIS
NUCLEIC ACIDS
OXIDATION
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
REDUCTION
RIBONUCLEIC ACID
VARIATIONS
X RADIATION