X-RAY EFFECTS ON CULTURED HUMAN AMNION CELLS *

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Annals of the New York Academy of Sciences

DOI:https://doi.org/10.1111/j.1749-6632.1961.tb50075.x· OSTI ID:4805915

Fogh, Jørgen; Lee Biedler, June; Denues, A. R. T.

The early effects of 100 kv x rays on monolayers of continuously grown human amnion cells of strain FL include loss of continuity of the cell sheet, reduction in number of cells, gradual increase in cell size, and decrease in occurrence of mitotic figures. For doses examined in the range 300 to 3000 r, the increase in cell size was accompanied by a greater range of volumes. Cell enlargement, interpreted as continued cellular growth under the condition of inhibited cell division, was reflected in the nucleus as well as in the cytoplasm. The largest giant cells were present 4 days after irradiation. Mitotic figures did not occur in giant cells. Cytoplasmic changes in the irradiated cells included enlargement, vacuolization, and formation of empty-looking, rather structureless areas, mainly perinuclear, that may figure in the separation of cytoplasm and nuclei. Prominent ergastoplasm and well-preserved mitochondria and Golgi complexes were seen within the irradiated cells, but a bubbling at the cell surface with suggested blebs containing well-preserved endoplasmic reticulum suggested a sudden dissolution of the cell. Nuclear anomalies included increased number of nuclei as well as marked changes in size and shape. Budding and break- off of nuclear material, wrinkling, and irregularities of nuclear membrane lobulation, and vacuolization were frequently observed. The multinucleate cells occurred frequently and, apparently as a late stage, the nuclei seemed to explode into numerous spheroidal masses. The nuclear RNA was seen to take all sizes and, especially at the earlier stages of change, was characteristically in the form of irregular enlarged nucleoli. A central zone of relatively structureless material was often observed within the nucleoli and some evidence of their unraveling was seen. In multinucleate cells, small nucleoli were seen within some micronuclei. Surviving cells from cultures exposed once or twice to 1000 r showed no striking difference in growth behavior of x-ray resistance from the parent culture. The chromosomes of FL cells displayed responses to radiation that ranged from simple breakage to marked fragmentation, with increased dose of radiation, and included complicated structural rearrangements.

• Research Organization: Sloan-Kettering Inst. for Cancer Research, New York; and Cornell Univ. Medical Coll., New York
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-026751
• OSTI ID: 4805915
• Journal Information: Annals of the New York Academy of Sciences, Journal Name: Annals of the New York Academy of Sciences Journal Issue: 2 Vol. 95; ISSN 0077-8923
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMNION CELLS
ANIMAL CELLS
BIOLOGY AND MEDICINE
CELL NUCLEI
CHROMOSOMES
CYTOLOGY
MITOSIS
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RIBONUCLEIC ACID
X RADIATION