THE MECHANISM OF ACTION OF AET. III. THE EFFECT OF GAMMA RADIATION ON THE VISCOSITY AND THE ENZYMATIC ACTIVITY OF UREASE SOLUTIONS

Journal Article · Wed Nov 01 04:00:00 EST 1961 · Radiation Research

DOI:https://doi.org/10.2307/3571140· OSTI ID:4819911

Dickens, E A; Shapiro, B

Solutions containing urease, urease and guanidoethyl sulfide (GES)/sub 2/ , and urease-SEG in 0.047 M sodium phosphate buffer at pH 7.5 were given doses of up to 750,000 r with Co(sup 60) GAMMA rays. The specific viscosity of urease decreases initially and then levels off at about three-fourths the original value. Protection against these irradiation-produced viscosity changes occurs in urease solutions containing (GES)(sub 2), but not in solutions containing only urease-SEG. The enzymatic activity of urease solutions decreases as a resupt of GAMMA radiation. Addition of (GES(sub 2), inhibits the enzyme; however, in solutions containing only urease--SEG, the enzymatic activity is partially restored by gamma radiation. Irradiation of solutions of urease containing (GES), almost completely destroys the enzymatic activity of these solutions. The viscosity changes in irradiated urease solutions are interpreted as indicatlng intramolecular rearrangement. Two ways in which (GES)(sup 2), could prevent this rearrangement have been indicated. The mechanism of the reactivation of urease-SEG solutions by irradiation is also discussed. (auth)

• Research Organization: Albert Einstein Medical Center, Philadelphia
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-001638
• OSTI ID: 4819911
• Journal Information: Radiation Research, Journal Name: Radiation Research Vol. Vol: 15
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AET
BIOLOGY AND MEDICINE
CHEMICALS
COBALT 60
DECOMPOSITION
ENZYMES
GAMMA RADIATION
QUANTITY RATIO
RADIATION DOSES
RECOVERY
SOLUTIONS
UREA
UREASE
VISCOSITY