THE ROLE OF PROTEINS AND OF THE NUCLEIC ACIDS IN RESTORING THE REGENERATIVE CAPACITY OF THE EXTREMITIES IN SALAMANDERS (AXOLOTLS), WHICH HAD BEEN SUPPRESSED BY X-RAY IRRADIATION

Journal Article · Fri Jun 01 04:00:00 EDT 1962 · Doklady Akad, Nauk S.S.S.R.

OSTI ID:4805350

Polezhaev, L V; Teplits, N A; Tuchkova, S Ya

White and black salamanders 8 to 10 months old were given an x-ray dose of 10,000 r to the extremities, or a whole body dose of 1000 r. Hoth feet were then amputated 17 to l9 days-after the local irradiation. The salamanders were injected once a day for six days with 3 Mg of RNA, 2 mg DNA, 110 mg of liver protein, or 10 mg of acid protein from milk. In the experiments with local irradiation of the extremities the following series of experiments were performed with l0 salamanders per series: controls with simple irradiation and amputation: irradiation, amputation and treatment with RNA; the same treatment with DNA; the same treatment with acid milk protein; the same treatment with liver protein; the same treatment with RNA and liver protein; the same treatment with RNA and acid protein; treatment with RNA, and irradiation and amputution two months later; irradiation, treatment with liver protein, and amputation two months later; and irradiation, treatment with RNA, and amputation two months later. The extremities grew back on the salamanders in 5% of the cases for the controls, in 33% of the cases after treatment with RNA, in 22% of the cases after treatment with acid milk protein and in 12.5% of the cases after treatment with liver protein. On repeating the amputation of the extremity, treatment with RNA was especially effective in bringing about the growth of new limbs (90 to 100% of the cases). Biochemical analysis showed that the content of RNA in the irradiated limb fell from a normal of 400 gamma to l30 gamma per 100 mg of dry material, and the RNA content rises sharply when new limb growth takes place. (TTT)

• Research Organization: A.N. Severtsov Inst. of Animal Morphology, USSR
• NSA Number: NSA-16-026794
• OSTI ID: 4805350
• Journal Information: Doklady Akad, Nauk S.S.S.R., Journal Name: Doklady Akad, Nauk S.S.S.R. Vol. Vol: 144
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

ANIMALS
AXOLOTL
BIOCHEMISTRY
BIOLOGY AND MEDICINE
NUCLEIC ACIDS
PROTEINS
QUANTITY RATIO
RADIATION DOSES
REGENERATION
RIBONUCLEIC ACID
X RADIATION