VARIATION IN THE CONTENT OF NUCLEIC ACIDS IN THE EXTREMITIES OF AXOLOTLS AFTER IRRADIATION WITH X-RAYS AND AFTER RE-ESTABLISHMENT OF THE ABILITY TO REGENERATE THE EXTREMITIES

Journal Article · Thu Nov 01 04:00:00 EST 1962 · Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals

OSTI ID:4743800

Teplits, N A

A total of 2158 white and black axolotls 10 months in age were given an x-ray dose of 7000 to 10,000 r to both rear extremities after covering the body with a lead sheet 4 mm thick The first amputation was made 15 to 21 days after the irradiation. A total of 6 or 7 injections were then made daily in the right rear limb in order to re-establish the regeneration of the limb. The several limbs were weighed and analyzed for the RNA and DNA contents. Data are presented on the variation of RNA and DNA contert under the following experimental conditions: (1) normal, unirradiated, amputation, and treatment with various preparations; (2) control-simple irradiation and amputation; (3) irradiation, amputation, and treatment with 125 mu g RNA; (4) irradiation, amputation, and treatment with 85 mu g DNA; (5) irradiation, amputation, and treatment with 27.5 mgs of rat liver protein; (6) irradiation, amputation, and treatment with 10 mgs of acid milk protein; (7) irradiation, amputation, and treatment with RNA and liver protein; (8) irradiation, amputation, and treatment with RNA and acid protein; (9) irradiation, treatment with RNA, and amputation two months later; (10) irradiation, treatment with liver protein, and amputation two months later; (11) treatmert with RNA, irradiation two months later, and amputation; (12) irradiation, amputation, and treatment with liver cell nuclei containing some cytoplasma; (13) irradiation, amputation and treatment with pure cell nuclei; (14) irradiation, amputation, and treatment with lyophilized bone marrow; (15) irradiation, amputation, and treatment with a suspension of lyophilized spleen tissue, and (16) irradiation, amputation, and treatment with lyophilized muscle tissue. The results show that irradiation slows down the regenerative ability of the limbs and that the RNA contert drops from a normal value of 400 to 230 mu g per 100 mgs dry weight, while the DNA content drops from a normal value of 240 to 190 mu g per 100 mgs dry weight. As the limbs regain their regenerative capacity, the RNA and DNA cortents approach the normal values after the first and second amputations. Treatment with the biological preparations increased the RNA and DNA contents in the newly formed limbs by 20 to 25%. (TTT)

• Research Organization: Severtsov Inst. of Animal Morphology, Academy of Sciences, USSR
• NSA Number: NSA-17-007834
• OSTI ID: 4743800
• Journal Information: Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals, Journal Name: Doklady Akademii Nauk SSSR (U.S.S.R.) English translation currently published in a number of subject-oriented journals Vol. Vol: 147; ISSN DANKA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

ANIMALS
AXOLOTL
BIOLOGY AND MEDICINE
BONE MARROW
HEAD
LIVER
MUSCLES
NUCLEIC ACIDS
PROTEINS
QUANTITY RATIO
RADIATION DOSES
REGENERATION
RIBONUCLEIC ACID
SHIELDING
SPLEEN
SURGERY
X RADIATION