A SURVEY OF THE EFFECT OF METABOLIC ANTAGONISTS ON THE SYNTHESIS OF ANTIBODY IN AN IN VITRO SYSTEM

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Immunology (England)

OSTI ID:4795497

Dutton, R W; Pearce, J D

Rabbits previously immunized to 2 antigens (ovalbumin and horse gamma - globulin) were restimulated by injection of 1 antigen followed, 1 day later, by the 2nd. A cell suspension of their spleens was prepared 2 days after injection of the 2nd antigen and the effects of x irradiation and metabolic antagonists on antibody synthesis were studied. Control spleen cells synthesized both antibodies simultaneously when incubated in vitro. Irradiation of the cells before incubation inhibited antibody synthesis; the required dose for 50% inhibition varied from 120 to 800 rads. Irradiation markedly reduced cell viability, as little as 50 rads reducing cell survival to 48 hr to less than half that of nonirradiated cells. Cells from different rabbits showed wide variations in radiosensitivity. It was considered that radiation inhibited antibody synthesis as a result of cell death. A similar cause of inhibition was apparent with cells treated with mitomycin C, ethidium bromide, 6mercaptopurine, 5,6- dichlorobenzimidazole riboside, and 6-thioguanosine, but a more selective inhibition was produced by thymidine analogs, 6-azauridine, and colchicine. (H.H.D.)

• Research Organization: Postgraduate Medical School, London
• NSA Number: NSA-16-028784
• OSTI ID: 4795497
• Journal Information: Immunology (England), Journal Name: Immunology (England) Vol. Vol: 5; ISSN IMMUA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALBUMINS
ALKALOIDS
AMINES
ANIMAL CELLS
ANTIBIOTICS
ANTIGENS
ANTIMITOTIC DRUGS
BENZIMIDAZOLES
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CHLORINATED HYDROCARBONS
COLCHICINE
DRUGS
GLOBULINS
HETEROCYCLICS
IMIDAZOLES
IMMUNITY
LIFETIME
METABOLISM
MITOMYCIN
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENYL RADICALS
PURINES
QUANTITY RATIO
RABBITS
RADIATION DOSES
RADIATION EFFECTS
RADIOSENSITIVITY
SPLEEN
SUGARS
X RADIATION