RECOVERY OF LETHALLY IRRADIATED DOGS FOLLOWING INFUSION OF AUTOLOGOUS MARROW STORED AT LOW TEMPERATURE IN DIMETHYLSULPHOXIDE

Journal Article · Sat Dec 01 04:00:00 EST 1962 · Blood (U.S.)

OSTI ID:4788405

Cavins, J A; Kasakura, S; Thomas, E D; Ferrebee, J W

Sixteen normal young dogs were exposed to 1200 r of Co/sup 60/ radiation over their entire bodies continuously at 5 to 6 r per minute. They were then given intravenous infusions of 1 to 5 btllion of their own nucleated marrow cells that had been previously aspirated and stored frozen at --80 deg C in dimethylsulfoxide. Nine received samples stored in 10% dimethylsulfoxide; all made prompt clinical and hematoiogic recoveries. Three dogs received samples stored in 5% dimethylsulfoxide; one died. Three received samples stored in 15% dimethylsulfoxide; two died, No toxicity of dimethylsulfoxide was recognized. A disagreeable odor emanated from the injected animals and persisted for a number of hours. Dimethylsulfoxide in 10% concentration was found to be suitable for the preservation of dog marrow at low temperature. (auth)

• Research Organization: Mary Imogene Bassett Hospital, Cooperstown, N.Y.
• NSA Number: NSA-17-001346
• OSTI ID: 4788405
• Journal Information: Blood (U.S.), Journal Name: Blood (U.S.) Vol. Vol: 20; ISSN BLOOA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
BIOLOGY AND MEDICINE
BLOOD CELLS
BODY
BONE MARROW
CHEMICALS
COBALT 60
DOGS
GAMMA RADIATION
LETHAL DOSE
LOW TEMPERATURE
ODOR
QUANTITY RATIO
RADIATION DOSES
RECOVERY
SOLUTIONS
STORAGE
SULFOXIDES
TOXICITY
TRANSPLANTS