Kinetics of Megacaryocyte Proliferation.
The kinetics of the megaca-ryocytic cell line of the rat bone marrow were studied using tritiated thymidine as a cell label. The changes in the percentage of labeled cells as a function of time after injection of the tracer were registered separately for arbitrarily chosen successive recognizable stages of megacaryocytic differentiation. Emphasis is put on the development of initially labeled cells into a stage of maturation corresponding to initially non- labeling cell forms. The transit time for the most immature recognizable stage of megacaryocytic development to megacaryocytic disintegration is approximately 40 hours. Evidence was obtained that the recognizable megacaryocytic elements originate from unrecognized precursors which continuously synthesize DNA for a period of at least 1 to 3 days prior to maturation into recognizable megacaryocytic precursors. The immature megacaryocytic cells able to synthesize DNA take up more H/sup 3/ thymidine than the rest of the bone marrow cells. This is consistent with polyploidy. The process of nuclear lobulation is not acoomplished by the end of DNA synthesis, thus being comparable to nuclear segmentation in neutrophilic granulocytes. This latest phase of maturation is relatively long (approximately 25 to 30 hours) as compared to the phase during which recognizable megacaryocytic precursors which recognizable megacaryocytic precursors are able to synthesize DNA (less than 15 hours). (auth)
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.
- Sponsoring Organization:
- US Atomic Energy Commission (AEC)
- DOE Contract Number:
- AT(30-2)-GEN-16
- NSA Number:
- NSA-16-032680
- OSTI ID:
- 4781672
- Report Number(s):
- BNL-6275
- Journal Information:
- Experimental Biology and Medicine, Journal Name: Experimental Biology and Medicine Journal Issue: 1 Vol. 111; ISSN 1535-3702
- Country of Publication:
- United States
- Language:
- English
Similar Records
THE EFFECTS OF RADIATION ON TOTAL AND DNA SYNTHESIZING CELLS OF THE BONE MARROW AND BLOOD
STABILITY OF RNA AND DNA IN BONE MARROW CELLS, STUDIED WITH TRITIATED CYTIDINE AND THYMIDINE